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तीव्र मस्तिष्क स्लाइसें में neuronal microcircuits के electrophysiological और morphological विशेषता बनती पैच दबाना रिकॉर्डिंग का उपयोग
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Capítulos
- 00:05Título
- 02:22Paired Patch-Clamping: Neuronal Connections with a Low Connectivity
- 05:11Paired Patch-Clamping: Neuronal Connections with a High Connectivity
- 06:16Neuronal Reconstruction and Synaptic Contact Localization
- 07:17Results: Morphology and Electrophysiology of Synaptic Connections in Cortical Neuronal Microcircuits
- 09:35Conclusion
Patch-clamp recordings and simultaneous intracellular biocytin filling of synaptically coupled neurons in acute brain slices allow a correlated analysis of their structural and functional properties. The aim of this protocol is to describe the essential technical steps of electrophysiological recording from neuronal microcircuits and their subsequent morphological analysis.
