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Электрофизиологических и морфологических характеристик нейронов микросхем при остром мозга срезов при использовании парных патч-зажим Recordings
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Electrophysiological and Morphological Characterization of Neuronal Microcircuits in Acute Brain Slices Using Paired Patch-Clamp Recordings
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Электрофизиологических и морфологических характеристик нейронов микросхем при остром мозга срезов при использовании парных патч-зажим Recordings

16,918 Views

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10:24 min

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January 10, 2015

DOI:

10.3791/52358-v

DOI:
10.3791/52358-v

•

10:24 min
January 10, 2015

•

3 Views
, ,
1Institute of Neuroscience and Medicine (INM-2),Research Centre Jülich, 2Department of Psychiatry, Psychotherapy and Psychosomatics, Medical Faculty, JARA,RWTH Aachen University

Summary

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Patch-clamp recordings and simultaneous intracellular biocytin filling of synaptically coupled neurons in acute brain slices allow a correlated analysis of their structural and functional properties. The aim of this protocol is to describe the essential technical steps of electrophysiological recording from neuronal microcircuits and their subsequent morphological analysis.

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Paired Patch-clamp RecordingAcute Brain SliceNeuronal MicrocircuitsSynaptic TransmissionElectrophysiologyMorphologyConnectivityChemical SynapsesGap JunctionsNeuron ReconstructionDendritic TruncationAxonal Truncation

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Qi, G., Radnikow, G., Feldmeyer, D. Electrophysiological and Morphological Characterization of Neuronal Microcircuits in Acute Brain Slices Using Paired Patch-Clamp Recordings. J. Vis. Exp. (95), e52358, doi:10.3791/52358 (2015).

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