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December 27, 2014
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Summary
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Here we present a protocol that generates large amounts of murine monocytes from heterogeneous bone marrow for translational applications. In comparison to others, this new method helps reduce the number of sacrificed animals and lowers costs by avoiding expensive methods such as high gradient magnetic cell separation (MACS).
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Wagner, M., Koester, H., Deffge, C., Weinert, S., Lauf, J., Francke, A., Lee, J., Braun- Dullaeus, R. C., Herold, J. Isolation and Intravenous Injection of Murine Bone Marrow Derived Monocytes. J. Vis. Exp. (94), e52347, doi:10.3791/52347 (2014).
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