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Primer for Immunhistokjemi på Cryosectioned Rat hjernevev: Eksempel Farging for Microglia og Nevroner
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Neurociencias
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JoVE Revista Neurociencias
Primer for Immunohistochemistry on Cryosectioned Rat Brain Tissue: Example Staining for Microglia and Neurons

Primer for Immunhistokjemi på Cryosectioned Rat hjernevev: Eksempel Farging for Microglia og Nevroner

DOI:
10.3791/52293-v

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07:30 min

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May 12, 2015

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, , ,
1Department of Child Health,University of Arizona College of Medicine – Phoenix, 2BARROW Neurological Institute,Phoenix Children’s Hospital, 3Department of Biology and Biochemistry,University of Bath, 4Neuroscience Program,Arizona State University, 5Phoenix VA Healthcare System

Capítulos

  • 00:05Título
  • 01:17Slide Processing and Tissue Staining
  • 04:58Fluorescent Imaging of the Stained Tissue
  • 05:55Results: The Good and the Bad: Fluorescent Images Iba-1/Pan-neuronal Double-labelling
  • 07:03Conclusion

Summary

Traducción Automática

Traducción Automática

This introductory level protocol describes the reagents, equipment, and techniques required to complete immunohistochemical staining of rodent brains, using markers for microglia and neuronal elements as an example.

Tags

ImmunohistochemistryCryosectionedRat BrainMicrogliaNeuronsFluorescenceDouble-labelingIba1Pan-neuronalVisualizationProtein Co-localizationDABAlkaline Phosphatase

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