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Optimizing Attachment of Human Mesenchymal Stem Cells on Poly(ε-caprolactone) Electrospun Yarns
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Capítulos
- 00:05Título
- 01:28Scaffold Set-up
- 03:10Cell Seeding and Experimental Start
- 04:43DNA Assay
- 06:44Scanning Electron Microscope (SEM) Fixation
- 08:49Results: Location of Cells Identified Following Four Hours of Culture
- 10:16Conclusion
This article describes a range of set-ups for seeding human mesenchymal stem cells onto materials, in this case electrospun yarns, that do not cover the base of standard culture well plates in order to maximize and quantify the number of cells that initially attach compared to the known seeding density.
