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プロ蛍光基質アナログを経由してPAD4活性の蛍光ベースの監視
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November 05, 2014
DOI:
Summary
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PAD4 is an enzyme responsible for the conversion of peptidyl-arginine to peptidyl-citrulline. Dysregulation of PAD4 has been implicated in a number of human diseases. A facile and high-throughput compatible fluorescence based PAD4 assay is described.
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Sabulski, M. J., Fura, J. M., Pires, M. M. Fluorescence-based Monitoring of PAD4 Activity via a Pro-fluorescence Substrate Analog. J. Vis. Exp. (93), e52114, doi:10.3791/52114 (2014).
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