Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Fluorescence-based Monitoring of PAD4 Activity via a Pro-fluorescence Substrate Analog
9,722 Views
•
08:37 min
•
November 05, 2014
DOI:
Summary
Automatically generated
PAD4 is an enzyme responsible for the conversion of peptidyl-arginine to peptidyl-citrulline. Dysregulation of PAD4 has been implicated in a number of human diseases. A facile and high-throughput compatible fluorescence based PAD4 assay is described.
Read Article
Cite this Article
Sabulski, M. J., Fura, J. M., Pires, M. M. Fluorescence-based Monitoring of PAD4 Activity via a Pro-fluorescence Substrate Analog. J. Vis. Exp. (93), e52114, doi:10.3791/52114 (2014).
Copy