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肾再生的成年斑马鱼庆大霉素性损伤
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Biología del desarrollo
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JoVE Revista Biología del desarrollo
Kidney Regeneration in Adult Zebrafish by Gentamicin Induced Injury
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肾再生的成年斑马鱼庆大霉素性损伤

10,600 Views

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06:25 min

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August 03, 2015

DOI:

10.3791/51912-v

DOI:
10.3791/51912-v

•

06:25 min
August 03, 2015

•

9 Views
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1Nephrology Division, Department of Medicine,Massachusetts General Hospital, 2Basic Sciences Division,Fred Hutchinson Cancer Research Center, 3Department of Genetics,Harvard Medical School

Summary

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Here we present a reliable method to study adult kidney regeneration by inducing acute kidney injury by gentamicin injection. We show that injury is dependent on gentamicin dosage and environmental temperature using in situ hybridization to label lhx1a+ developing new nephrons.

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Cite this Article

Kamei, C. N., Liu, Y., Drummond, I. A. Kidney Regeneration in Adult Zebrafish by Gentamicin Induced Injury. J. Vis. Exp. (102), e51912, doi:10.3791/51912 (2015).

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