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मॉडलिंग तारिकाकोशिकार्बुद रोगजनन<em> इन विट्रो</em> और<em> इन विवो</em> Cortical Astrocytes या सशर्त, आनुवंशिक रूप से इंजीनियर चूहों से तंत्रिका स्टेम कोशिकाओं का उपयोग
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1Department of Pathology and Laboratory Medicine,University of North Carolina School of Medicine, 2Lineberger Comprehensive Cancer Center,University of North Carolina School of Medicine, 3Division of Neuropathology, Department of Pathology and Laboratory Medicine,University of North Carolina School of Medicine, 4Curriculum in Genetics and Molecular Biology,University of North Carolina School of Medicine, 5Biological and Biomedical Sciences Program,University of North Carolina School of Medicine, 6Department of Radiation Oncology,Emory University School of Medicine, 7Department of Neurology, Neurosciences Center,University of North Carolina School of Medicine
Capítulos
- 00:05Título
- 01:45Tissue Harvest
- 02:49Tissue Homogenization
- 04:55Cortical Astrocytes Preparation for Intracranial Injection
- 06:22Orthotopic Implantation
- 07:56Results: Representative Orthopically Implanted Cortical Astrocytes
- 09:31Conclusion
Phenotypically wild-type astrocytes and neural stem cells harvested from mice engineered with floxed, conditional oncogenic alleles and transformed via viral Cre-mediated recombination can be used to model astrocytoma pathogenesis in vitro and in vivo by orthotopic injection of transformed cells into brains of syngeneic, immune-competent littermates.
