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Immunology and Infection

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JoVE Journal Immunology and Infection
High-throughput Assay to Phenotype Salmonella enterica Typhimurium Association, Invasion, and Replication in Macrophages
 

High-throughput Assay to Phenotype Salmonella enterica Typhimurium Association, Invasion, and Replication in Macrophages

Article DOI: 10.3791/51759-v • 11:10 min • August 11th, 2014
August 11th, 2014 •
1, 1, 1, 2, 3, 4, 1
1Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, 2Department of Microbial and Molecular Pathogenesis, College of Medicine, Texas A&M University System Health Science Center, 3University of California, Irvine, 4Department of Medical Microbiology & Immunology, School of Medicine, University of California, Davis

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A high-throughput assay to in vitro phenotype Salmonella or other bacterial association, invasion, and replication in phagocytic cells with high-throughput capacity was developed. The method was employed to evaluate Salmonella gene knockout mutant strains for their involvements in host-pathogen interactions.

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High-throughput Assay Phenotype Salmonella Enterica Typhimurium Association Invasion Replication Macrophages Zoonotic Pathogens Food Borne Illnesses Molecular Pathogenesis Gentamicin Protection Assay Deletion Mutants Salmonella-host Interactions RAW 264.7 Murine Macrophages Multichannel Pipettes Precision 96-well Culture Dish In Vitro Salmonella Invasion Assay
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