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Assessment of Ovarian Cancer Spheroid Attachment and Invasion of Mesothelial Cells in Real Time
Capítulos
- 00:05Título
- 02:05Optional Fluorescent Cell Labeling Method
- 03:24Generation of Spheroids in Methylcellulose
- 04:28Real Time Cell Analyzer (RTCA) Cell Invasion Assay
- 05:46Programming the RTCA Instrument
- 07:25Generation of LP9 Monolayer and Measurement of Spheroid Invasion
- 09:49Results: Real Time Assessment of Ovarian Cancer Spheroid Invasion
- 13:42Conclusion
Ovarian cancer cell invasion into the mesothelial lining of the peritoneum is a dynamic process over time. Utilizing a real time analyzer, the invasive capacity of ovarian cancer cells in a spheroid-mesothelial cell co-culture model can be quantified over prolonged time periods, providing insights into factors regulating the metastatic process.
