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Hot Biological Catalysis: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions
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April 04, 2014
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Isothermal titration calorimetry measures heat flow released or absorbed in chemical reactions. This method can be used to quantify enzyme-catalysis. In this paper, the protocol for instrumental setup, experiment running, and data analysis is generally described, and applied to the characterization of enzymatic urea hydrolysis by jack bean urease.
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Mazzei, L., Ciurli, S., Zambelli, B. Hot Biological Catalysis: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions. J. Vis. Exp. (86), e51487, doi:10.3791/51487 (2014).
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