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A Simple and Rapid Protocol for Measuring Neutral Lipids in Algal Cells Using Fluorescence
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A Simple and Rapid Protocol for Measuring Neutral Lipids in Algal Cells Using Fluorescence

A Simple and Rapid Protocol for Measuring Neutral Lipids in Algal Cells Using Fluorescence

35,043 Views

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10:56 min

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May 30, 2014

DOI:

10.3791/51441-v

DOI:
10.3791/51441-v

•

10:56 min
May 30, 2014

•

8 Views
, , ,
1Department of Chemical and Materials Engineering,University of Alberta, 2Department of Chemical and Petroleum Engineering,University of Calgary

Summary

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A simple protocol to determine the neutral lipid content of algal cells using a Nile Red staining procedure is described. This time-saving technique offers an alternative to traditional gravimetric-based lipid quantification protocols. It has been designed for the specific application of monitoring bioprocess performance.

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Algal CellsNeutral LipidsFluorescenceNile RedAuxenochlorella ProtothecoidesLipid ContentBioprocess MonitoringRapid ProtocolGravimetric MethodsCell Membrane Permeabilization

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Cite this Article

Storms, Z. J., Cameron, E., de la Hoz Siegler, H., McCaffrey, W. C. A Simple and Rapid Protocol for Measuring Neutral Lipids in Algal Cells Using Fluorescence. J. Vis. Exp. (87), e51441, doi:10.3791/51441 (2014).

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