The protocol described herein aims to explain and abridge the numerous obstacles in the way of the intricate route leading to modified nucleoside triphosphates. Consequently, this protocol facilitates both the synthesis of these activated building-blocks and their availability for practical applications.
Hollenstein, M., Smith, C. C., Räz, M. Nucleoside Triphosphates - From Synthesis to Biochemical Characterization. J. Vis. Exp. (86), e51385, doi:10.3791/51385 (2014).