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Neonatal Pial Surface Electroporation
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Neonatal Pial Surface Electroporation

Neonatal Pial Surface Electroporation

13,708 Views

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06:22 min

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May 07, 2014

DOI:

10.3791/51319-v

DOI:
10.3791/51319-v

•

06:22 min
May 07, 2014

•

2 Views
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1Department of Neurosurgery, Regenerative Medicine Institute,Cedars-Sinai Medical Center, 2Department of Biomedical Sciences, Regenerative Medicine Institute,Cedars-Sinai Medical Center

Summary

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The pial surface is a unique progenitor zone in the CNS that is receiving increasing attention. Herein, we detail a method for rapid genetic manipulation of this progenitor zone using a modified electroporation method. This procedure can be used for cellular and molecular investigations of cell lineages and signaling pathways involved in cell differentiation and to elucidate the fate and properties of daughter cells.

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Pial SurfaceElectroporationNeonatalProgenitorNeural Stem CellGenetic ManipulationGliogenesisNeurogenesisEmbryonicPostnatal

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Cite this Article

Levy, R., Molina, J., Danielpour, M., Breunig, J. J. Neonatal Pial Surface Electroporation. J. Vis. Exp. (87), e51319, doi:10.3791/51319 (2014).

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