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Immunology and Infection

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JoVE Journal Immunology and Infection
Assessing the Innate Sensing of HIV-1 Infected CD4+ T Cells by Plasmacytoid Dendritic Cells Using an Ex vivo Co-culture System.
 

Assessing the Innate Sensing of HIV-1 Infected CD4+ T Cells by Plasmacytoid Dendritic Cells Using an Ex vivo Co-culture System.

Article DOI: 10.3791/51207-v • 08:11 min • September 1st, 2015
September 1st, 2015 •
1, 1, 1,2
1Laboratory of Human Retrovirology, Institut de Recherches Cliniques de Montréal (IRCM), 2Department of Microbiology, Infectiology, and Immunology, Université de Montréal

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Resumen
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Unlike cell-free HIV-1 particles, infected CD4+ T cells are effectively sensed by plasmacytoid dendritic cells (pDCs). This manuscript describes a method where peripheral blood mononuclear cells (PBMCs) or isolated pDCs are co-cultured with HIV-1 infected T cells to evaluate innate sensing by pDCs as assessed by release of type-I IFN.

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Innate Sensing HIV-1 Infected CD4+ T Cells Plasmacytoid Dendritic Cells Ex Vivo Co-culture System PBMCs T Cell Line MT4 Primary CD4+ T Cells Flow Cytometric Staining Surface Markers Toll-Like Receptors (TLR) Pathways Type-I Interferon (IFN) Production 96 Well Plates Bioactive Type-I IFNs ISGF3 Pathway
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