Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
1Department of Biomedical and Molecular Sciences,Queen’s University, 2National Institute of Neurological Disorders and Stroke,Porter Neuroscience Research Center, 3Research Institute of Genome-Based Biofactory,National Institute of Advanced Industrial Science and Technology, 4The Robert H. Smith Faculty of Agriculture, Food and Environment, Institute of Biochemistry, Food Science, and Nutrition,The Hebrew University of Jerusalem
Capítulos
- 00:05Título
- 01:14Growing Single Ice Crystals
- 03:18Determining Singularity and Orientation o the Ice Crystal
- 04:37Absorption of Fluorescently Labeled Antifreeze Proteins to a Single Ice Crystal
- 07:20Results: Fluorescence-based Ice Plane Affinity Analysis Images
- 08:18Conclusion
Frostvæskeproteiner (AFP'er) binder sig til specifikke isplaner for at forhindre eller bremse isvækst. Fluorescens-baserede isplan affinitet (FIPA) analyse er en ændring af den oprindelige is-ætsning metode til bestemmelse af AFP-bundet is planer. AFP'er er fluorescerende mærket, indarbejdet i makroskopiske enkeltiskrystaller og visualiseret under UV-lys.
