Journal
/
Inmunología y contagio
/
2D and 3D Chromosome Painting in Malaria Mosquitoes
JoVE Revista
Inmunología y contagio
This content is Free Access.
JoVE Revista Inmunología y contagio
2D and 3D Chromosome Painting in Malaria Mosquitoes
Please note that all translations are automatically generated. Click here for the English version.

2D and 3D Chromosome Painting in Malaria Mosquitoes

DOI:
10.3791/51173-v

•

09:57 min

•

January 06, 2014

•

, ,
1Department of Entomology,Virginia Tech

Capítulos

  • 00:05Título
  • 01:11Overview of Isolating DNA from Polytene Chromosomes by Laser Capture Microdissection
  • 02:07Probe Preparation via Whole Genome Amplification
  • 03:593D FISH on Whole-mount Ovarian Tissue
  • 07:33WGA Kit Yields and FISH Labeling
  • 09:31Conclusion

Summary

Traducción Automática

Traducción Automática

Chromosome painting is a useful method for studying organization of the cell nucleus and evolution of the karyotype. Here, we demonstrate an approach to isolate and amplify specific regions of interest from single polytene chromosomes that are subsequently used for two- and three-dimensional fluorescent in situ hybridization (FISH).

Tags

Chromosome PaintingFISHLaser Capture MicrodissectionWhole Genome AmplificationAnopheles GambiaeMalaria MosquitoPolytene ChromosomeChromosome OrganizationCytogenetics

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

check_url/es/51173?article_type=v

A Simple Chelex Protocol for DNA Extraction from Anopheles spp.
check_url/es/51173?article_type=v

Fluorescent in situ Hybridization on Mitotic Chromosomes of Mosquitoes
check_url/es/51173?article_type=v

Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH
check_url/es/51173?article_type=v

Proteomic Profiling of Macrophages by 2D Electrophoresis
check_url/es/51173?article_type=v

Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria
check_url/es/51173?article_type=v

An In Vitro Model for Measuring Immune Responses to Malaria in the Context of HIV Co-infection
check_url/es/51173?article_type=v

Evaluation of Extracellular Vesicle Function During Malaria Infection
check_url/es/51173?article_type=v

Rescue of Recombinant Zika Virus from a Bacterial Artificial Chromosome cDNA Clone
check_url/es/51173?article_type=v

Vector Competence Analyses on Aedes aegypti Mosquitoes using Zika Virus
check_url/es/51173?article_type=v

Propagation of the Microsporidian Parasite Edhazardia aedis de Aedes aegypti Mosquitoes

Read Article