Journal
/
Inmunología y contagio
/
시각화 및 정량하기 위해 형광 단백질을 사용하여<em> 클라미디아</em> 액포 성장 역학 살아있는 세포에서
JoVE Revista
Inmunología y contagio
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Inmunología y contagio
Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells
Please note that all translations are automatically generated. Click here for the English version.

시각화 및 정량하기 위해 형광 단백질을 사용하여<em> 클라미디아</em> 액포 성장 역학 살아있는 세포에서

7,414 Views

•

07:42 min

•

October 13, 2015

DOI:

10.3791/51131-v

DOI:
10.3791/51131-v

•

07:42 min
October 13, 2015

•

2 Views
, ,
1Division of Allergy and Infectious Diseases, Department of Medicine,University of Washington, 2Program in Infectious Diseases, School of Public Health,University of California at Berkeley

Summary

Automatically generated

A live cell fluorescent protein based method for illuminating cellular vacuoles (inclusions) containing Chlamydia is described. This strategy enables rapid, automated determination of Chlamydia infectivity in samples and can be used to quantitatively investigate inclusion growth dynamics.

Explore More Videos

ChlamydiaInclusionVacuoleFluorescent ProteinsGFPMCherryVisualizationQuantitationHost CellIntracellularImagingReverse-imagingAntibody-based Enumeration

Read Article

Cite this Article

Zuck, M., Feng, C., Hybiske, K. Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells. J. Vis. Exp. (104), e51131, doi:10.3791/51131 (2015).

Download .ris file

Copy

Share Video

.

Copy