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检测现场的<em>大肠杆菌</em> O157：H7细胞的PMA-qPCR的

JoVE Revista
Biología

This content is Free Access.

JoVE Revista Biología

Detection of Live Escherichia coli O157:H7 Cells by PMA-qPCR

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检测现场的<em>大肠杆菌</em> O157：H7细胞的PMA-qPCR的

16,308 Views

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08:16 min

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February 01, 2014

DOI:

10.3791/50967-v

DOI:

10.3791/50967-v

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08:16 min

February 01, 2014

•

4 Views

Baoguang Li¹, Zonglin Hu¹, Christopher A. Elkins¹

¹Center for Food Safety and Applied Nutrition, Division of Molecular Biology,Food and Drug Administration

Summary

H7定位独特的遗传标记，Z3276：一个定量PCR法检测检测大肠杆菌 O157发展。在定量PCR是结合单叠氮丙啶（PMA）处理活细胞检测。这个协议已经被修改，并适于方便和一致地处理大量样品的96孔板格式

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