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Laser-scanning Photostimulation of Optogenetically Targeted Forebrain Circuits
JoVE Revista
Neurociencias
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JoVE Revista Neurociencias
Laser-scanning Photostimulation of Optogenetically Targeted Forebrain Circuits

Laser-scanning Photostimulation of Optogenetically Targeted Forebrain Circuits

DOI:
10.3791/50915-v

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07:43 min

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December 27, 2013

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, , ,
1Department of Comparative Biomedical Sciences, School of Veterinary Medicine,Louisiana State University, 2Department of Neurobiology,University of Chicago

Capítulos

  • 00:05Título
  • 01:29Surgery and Injections
  • 01:52Slice Preparation
  • 02:37Physiological Recording with Laser-scanning Photostimulation
  • 04:22Fixed Slice Imaging
  • 05:16Channelrhodopsin Expression in V1-V2 and S1-VP Projections
  • 07:07Conclusion

Summary

Traducción Automática

Traducción Automática

We describe a method for characterizing the functional topography and synaptic properties of forebrain circuits using an optogenetic approach to photostimulate neuronal populations in vitro.

Tags

Laser-scanning PhotostimulationOptogeneticsForebrain CircuitsChannelrhodopsinCre-lox RecombinationWhole-cell Patch ClampFunctional TopographySynaptic PropertiesConfocal Microscopy

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