We present methods to study the effect of PSMs and other toxins secreted by Staphylococcus aureus on neutrophils using flow cytometry and fluorescence microscopy.
Surewaard, B. G., van Strijp, J. A., Nijland, R. Studying Interactions of Staphylococcus aureus with Neutrophils by Flow Cytometry and Time Lapse Microscopy. J. Vis. Exp. (77), e50788, doi:10.3791/50788 (2013).