/
/
Culturing and Maintaining Clostridium difficile in an Anaerobic Environment
Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Culturing and Maintaining Clostridium difficile in an Anaerobic Environment
Capítulos
- 00:05Título
- 01:51Culturing, Enumerating, and Storing C. difficile from Stool Samples
- 05:59Culturing C. difficile from Frozen Glycerol Stocks
- 07:10Purifying Spores from C. difficile
- 09:12Results: C. difficile Successfully Grown on Various Media
- 10:16Conclusion
Clostridium difficile is a pathogenic bacterium that is a strict anaerobe and causes antibiotic associated diarrhea (AAD). Here, methods for isolating, culturing and maintaining C. difficile vegetative cells and spores are described. These techniques necessitate an anaerobic chamber, which requires regular maintenance to ensure proper conditions for optimal C. difficile cultivation.
