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打印温敏反向模具图案创作的双组分水凝胶的三维细胞培养
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture
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打印温敏反向模具图案创作的双组分水凝胶的三维细胞培养

14,901 Views

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10:49 min

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July 10, 2013

DOI:

10.3791/50632-v

DOI:
10.3791/50632-v

•

10:49 min
July 10, 2013

•

3 Views
, , ,
1Department of Health Science & Technology,Cartilage Engineering & Regeneration, 2Biomaterials Department,Innovent e.V.

Summary

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àbioprinter被用于创建图案的水凝胶的基础上牺牲的模具。泊洛沙姆模具与第二水凝胶回填，然后洗脱，留下空隙充满了三分之一的水凝胶。此方法使用快速的洗脱和良好的印刷泊洛沙姆产生复杂的生物大分子结构。

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Cite this Article

Müller, M., Becher, J., Schnabelrauch, M., Zenobi-Wong, M. Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture. J. Vis. Exp. (77), e50632, doi:10.3791/50632 (2013).

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