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的分散Presomitic中胚层细胞培养的斑马鱼分割时钟在单细胞成像代
JoVE Revista
Biología
This content is Free Access.
JoVE Revista Biología
Generation of Dispersed Presomitic Mesoderm Cell Cultures for Imaging of the Zebrafish Segmentation Clock in Single Cells
DOI:

10:41 min

July 24, 2014

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Capítulos

  • 00:05Título
  • 01:39Dissection Preparation
  • 03:15Presomitic Mesoderm Dissection and Dispersal
  • 06:40Imaging Dispersed Presomitic Mesodermal Cells
  • 07:58Image Processing of Acquired Time-lapse Movies
  • 08:58Representative Time-lapse Imaging Results
  • 10:00Conclusion

Summary

Traducción Automática

体节形成是有节奏的发育过程,从而在空间型态脊椎动物胚胎的体轴。以前,我们开发了使用荧光记者观察环状基因,推动这一进程的转基因斑马鱼线。在这里,我们培养从这些线和图像的振荡时间的推移在体外分散细胞。

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