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的分散Presomitic中胚层细胞培养的斑马鱼分割时钟在单细胞成像代
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July 24, 2014
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体节形成是有节奏的发育过程，从而在空间型态脊椎动物胚胎的体轴。以前，我们开发了使用荧光记者观察环状基因，推动这一进程的转基因斑马鱼线。在这里，我们培养从这些线和图像的振荡时间的推移在体外分散细胞。
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Webb, A. B., Soroldoni, D., Oswald, A., Schindelin, J., Oates, A. C. Generation of Dispersed Presomitic Mesoderm Cell Cultures for Imaging of the Zebrafish Segmentation Clock in Single Cells. J. Vis. Exp. (89), e50307, doi:10.3791/50307 (2014).
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