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JoVE Journal Neuroscience
Extracellularly Identifying Motor Neurons for a Muscle Motor Pool in Aplysia californica
 

Extracellularly Identifying Motor Neurons for a Muscle Motor Pool in Aplysia californica

Article DOI: 10.3791/50189-v • 13:37 min • March 25th, 2013
March 25th, 2013 •
1, 1, 1,2,3
1Department of Biology, Case Western Reserve University, 2Department of Neurosciences, Case Western Reserve University, 3Department of Biomedical Engineering, Case Western Reserve University

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In animals with large identified neurons (e.g. mollusks), analysis of motor pools is done using intracellular techniques1,2,3,4. Recently, we developed a technique to extracellularly stimulate and record individual neurons in Aplysia californica5. We now describe a protocol for using this technique to uniquely identify and characterize motor neurons within a motor pool.

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Extracellularly Identifying Motor Neurons Muscle Motor Pool Aplysia Californica Intracellular Techniques Extracellular Stimulation Extracellular Recording Uniquely Identify Motor Neurons Characterize Motor Neurons Advantages Of Extracellular Technique Ganglia Rotation Access Neurons On Both Sides Of The Ganglion Multiple Neurons Identification Less Damage To Neurons Flexible Extracellular Electrodes Muscle Movements
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