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Isolation and Culture of Individual Myofibers and their Satellite Cells from Adult Skeletal Muscle
Capítulos
- 00:05Título
- 01:44Preparations
- 03:33Muscle Dissection and Digestion
- 07:00Single Myofiber Dissociation and Culture
- 09:35Ex vivo Expression of Pax7 and Myf5 in Myofibers
- 11:02Conclusion
Isolation and culture of myofibers is the gold standard in vitro system to study the transition of satellite cells through quiescence, activation and differentiation. Importantly, the single myofiber culture system preserves the myofiber/stem cell association, which is an essential component of the muscle stem cell niche.
