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Capítulos
- 00:05Título
- 01:27Preparation of cDNA Expression Library
- 05:43Cell Seeding and Transfection
- 07:10Image Acquisition
- 08:51Results: Changes in Grb2 Localization by cDNA Expression
- 10:24Conclusion
Et højt indhold screeningsmetode til identifikation af hidtil ukendte signalering kompetente transmembrane receptorer er beskrevet. Denne metode er muligt at foretage en storstilet automatisering og giver mulighed for forudsigelser om<em> In vivo</em> Proteinbinding og den sub-cellulære lokalisering af protein-komplekser i pattedyrceller.
Tags
High-content ImagingGrb2 Signaling ComplexesExpression CloningSignal TransductionGrowth Factor ReceptorsDownstream Signaling CascadesMitogenic SignalingSH2 DomainSH3 DomainGTPase Son-Of-Sevenless (SOS)Phosphorylated Tyrosine ResiduesSOS-Ras-MAP Kinase Signaling CascadePositive And Negative Regulator Of SignalingReceptor EndocytosisMicroscopy AssayPlasma Membrane ReceptorsEpidermal Growth Factor Receptor (EGFR)GFP-Grb2
