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A High-content Imaging Workflow to Study Grb2 Signaling Complexes by Expression Cloning
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Biología
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JoVE Revista Biología
A High-content Imaging Workflow to Study Grb2 Signaling Complexes by Expression Cloning

A High-content Imaging Workflow to Study Grb2 Signaling Complexes by Expression Cloning

10,471 Views

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10:52 min

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October 30, 2012

DOI:

10.3791/4382-v

DOI:
10.3791/4382-v

•

10:52 min
October 30, 2012

•

2 Views
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1MRC LMCB,University College London, 2Center for Computational and Integrative Biology,Massachusetts General Hospital

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High-content ImagingGrb2 Signaling ComplexesExpression CloningSignal TransductionGrowth Factor ReceptorsDownstream Signaling CascadesMitogenic SignalingSH2 DomainSH3 DomainGTPase Son-Of-Sevenless (SOS)Phosphorylated Tyrosine ResiduesSOS-Ras-MAP Kinase Signaling CascadePositive And Negative Regulator Of SignalingReceptor EndocytosisMicroscopy AssayPlasma Membrane ReceptorsEpidermal Growth Factor Receptor (EGFR)GFP-Grb2

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Freeman, J., Kriston-Vizi, J., Seed, B., Ketteler, R. A High-content Imaging Workflow to Study Grb2 Signaling Complexes by Expression Cloning. J. Vis. Exp. (68), e4382, doi:10.3791/4382 (2012).

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