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En modifierad EPA Method 1623 som använder tangentiellt flöde Ihåliga fibrer Ultrafiltrering och Heat dissociation åtgärder för att upptäcka Waterborne<em> Cryptosporidium</em> Och<em> Giardia SPP.</em
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.
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En modifierad EPA Method 1623 som använder tangentiellt flöde Ihåliga fibrer Ultrafiltrering och Heat dissociation åtgärder för att upptäcka Waterborne<em> Cryptosporidium</em> Och<em> Giardia SPP.</em

19,949 Views

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12:11 min

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July 09, 2012

DOI:

10.3791/4177-v

DOI:
10.3791/4177-v

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12:11 min
July 09, 2012

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4 Views
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1National Exposure Research Laboratory,Office of Research and Development, US Environmental Protection Agency, 2Shaw Environmental & Infrastructure, 3Office of Ground Water and Drinking Water,US Environmental Protection Agency

Tags

Modified EPA Method 1623Tangential Flow Hollow-fiber UltrafiltrationHeat DissociationWaterborne CryptosporidiumGiardia Spp.ProtozoaDiarrheal Disease OutbreaksPrevalenceMonitoringUS Drinking Water SuppliesSample ConcentrationImmunomagnetic Separation ProcedureAcid Dissociation ProcedureImmunofluorescence Staining And EnumerationEnvirochek FiltersEnvirochek HV FiltersFilta-Max Filters

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Rhodes, E. R., Villegas, L. F., Shaw, N. J., Miller, C., Villegas, E. N. A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.. J. Vis. Exp. (65), e4177, doi:10.3791/4177 (2012).

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