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Capítulos
- 00:05Título
- 02:47Preparation of Aggresome Donor Cells: Transfection of mRFP- or eGFP-tagged Human Full-length DISC1
- 03:50Aggresome Purification from Transfected Donor Cells
- 06:43Treatment of Recipient SH-SYBY Cells with mRFP-DISC1 Aggresomes
- 08:13Labeling of Recombinant DISC1(598-785) with DyLight594
- 10:44Treatment of Recipient SH-SY5Y Cells with Labeled Recombinant DISC1(598-785) Protein
- 12:00Representative Invasion Assay Results
- 13:30Conclusion
Hücre kültüründen hücre içi, sitoplazmik tam uzunluklu protein DISC1 aggresomes ve bir etiketlenmiş, multimerik DISC1 rekombinant protein parçasının üretimi, saflaştırılması ve hücre istilası<em> E. coli</em> Tarif edilmektedir. Hücre invazivlik hücre kültürü ve nöronlar için alıcı hücreler için gösterilir<em> In vivo</em> Stereotaktik beyin inokülasyon sonrası.
Tags
Protein AggregationChronic Degenerative Brain ConditionsNeurodegenerative DiseasesAlzheimer’s DiseaseTauParkinson’s DiseaseAlpha-synucleinHuntington’s DiseasePolyglutamineHuntingtinProteostasisMisfolded ProteinsSporadic FormsFamilial FormsSchizophreniaCognitive DeficitDisrupted-in-schizophrenia 1 (DISC1)Scottish FamilyChronic Mental DiseaseInsoluble AggregatesSMRI CCSarkosyl-insoluble DISC1 ImmunoreactivityBiochemical FractionationDisease-associated Polymorphism S704CDISC1 Aggresomes
