This content is Free Access.
Capítulos
- 00:05Título
- 01:25Cross-linking Cells to Preserve RNA-Chromatin Interactions
- 02:58Cell Lysis
- 03:37Shearing DNA by Sonication
- 05:13Chromatin Isolation by RNA Purification (ChIRP)
- 08:43Results: Enrichment of Target Long Noncoding RNAs by ChIRP
- 10:34Conclusion
CHIRP est une nouvelle technique et rapide à la carte génomique des sites de liaison de l'ARN non codantes de longues (lncRNAs). La méthode tire parti de la spécificité des oligonucléotides carrelage anti-sens pour permettre le dénombrement des sites génomiques lncRNA assortis.
Tags
Chromatin Isolation By RNA PurificationChIRPLong Noncoding RNAsChromatin StatesDosage CompensationImprintingDevelopmental Gene ExpressionLncRNAsChromatin Modification ComplexesPolycomb Repressive Complex 2Histone H3 Lysine 27 TrimethylationH3K27me3Gene-specific FashionCis RegulationTrans RegulationDrosophila LncRNAs RoX1RoX2X Chromosome Binding SitesHuman LncRNA HOTAIRPRC2 OccupancyGenome-wide EffectsModular ScaffoldsTERC RNATelomerase ComplexH3K4 Demethylase ComplexRNA Occupancy At Chromatin
