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The CYP2D6 Animal Model: How to Induce Autoimmune Hepatitis in Mice
Capítulos
- 00:05Título
- 01:43Intravenous Injection of Ad-2D6 into the Opthalmic Sinus
- 02:31Mouse Liver Perfusion
- 04:20Isolation of Liver Lymphocytes
- 05:39Representative Liver Morphology and Histology in hCYP2D6-infected Mice
- 07:30T Cell Responsiveness in hCYP2D6-infected Mice
- 08:15Conclusion
Infection of mice with an Adenovirus expressing the major human autoantigen cytochrome P450 2D6 (hCYP2D6) recognized by sera of patients suffering from type 2 autoimmune hepatitis results in a persistent form of autoimmune-mediated liver disease characterized by extensive hepatitis, fibrosis and generation of a CYP2D6-specific immune response.
Tags
CYP2D6Animal ModelAutoimmune HepatitisMiceInductionLife ThreateningLiverAutoimmune DiseaseEtiologyHuman DiseaseMouse ModelAutoantigenAIH-2HCYP2D6TriggerLiver-kidney Microsomal AntibodiesLKM-1 AntibodiesAdenovirus ConstructLocal InflammationAutoimmunityIntravenous InjectionIntraperitoneal InjectionLong-lasting Autoimmune DamageLiver DiseaseProtocolHepatocyte DestructionAminotransferasesHCYP2D6 Antibodies
