Journal
/
Biología
/
In-vivo-Elektroporation von Morpholinos in die Adult Zebrafisch Retina
JoVE Revista
Biología
This content is Free Access.
JoVE Revista Biología
In vivo Electroporation of Morpholinos into the Adult Zebrafish Retina
Please note that all translations are automatically generated. Click here for the English version.

In-vivo-Elektroporation von Morpholinos in die Adult Zebrafisch Retina

18,794 Views

•

06:55 min

•

December 27, 2011

DOI:

10.3791/3603-v

DOI:
10.3791/3603-v

•

06:55 min
December 27, 2011

•

5 Views
, ,
1Departments of Anatomy and Cell Biology and Ophthalmology,Wayne State University School of Medicine, 2Department of Biological Sciences,University of Notre Dame , 3Center for Zebrafish Research,University of Notre Dame

Summary

Automatically generated

Eine Methode, bedingt Knockdown ein Zielprotein Ausdruck in der erwachsenen Zebrafisch Retina beschrieben, das geht intravitreal Injektion antisense Morpholinos und Elektroporation sie in der Netzhaut. Das resultierende Protein ist für mehrere Tage, die Prüfung des Proteins Rolle bei der Regeneration oder intakten Netzhaut ermöglicht klopfte.

Explore More Videos

In Vivo ElectroporationMorpholinosAdult Zebrafish RetinaInherited Eye DiseasesProgressive BlindnessRetinal NeuronsRegenerationRetinal Damage ModelsPhotoreceptorsInner Retinal NeuronsMüller GliaNeuronal ProgenitorsRetinal LayerDifferentiationCellular ResponsesMRNA Microarray AnalysisOcular DevelopmentCandidate Gene/protein

Read Article

Cite this Article

Thummel, R., Bailey, T. J., Hyde, D. R. In vivo Electroporation of Morpholinos into the Adult Zebrafish Retina. J. Vis. Exp. (58), e3603, doi:10.3791/3603 (2011).

Download .ris file

Copy

Share Video

.

Copy