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2 - 광자 현미경을 사용하여 마우스 Thymus의 Intravital 영상
JoVE Revista
Inmunología y contagio
Author Produced
This content is Free Access.
JoVE Revista Inmunología y contagio
Intravital Imaging of the Mouse Thymus using 2-Photon Microscopy
DOI:

10:08 min

January 07, 2012

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Capítulos

  • 00:01Título
  • 00:05Introduction
  • 01:15Animal preparation: Irradiation and bone marrow transfer
  • 03:16Animal preparation: Thymic transplantation
  • 04:58Animal preparation: Surgery to exposure transplanted thymus
  • 06:53Two-photon image acquisition
  • 08:35Resultados

Summary

Traducción Automática

We have developed novel laboratory tools and protocols for intravital imaging acquisition of the thymus. Our technique should help in the identification of “niches” within the thymus where T cell development occurs.

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