This content is Free Access.
Capítulos
- 00:05Título
- 01:20Automated Adherent Cell Culture Process
- 03:31shRNA Virus Production and Plating onto Assay Plates
- 05:44Lentiviral Transduction and Neuronal Differentiation of SH-SY5Y Cells
- 06:57High Content Image Acquisition and Image Analysis
- 09:27Representative Results of High Throughput Content Screening
- 13:09Conclusion
Vi beskriver en metode som kombinerer automatisert celledyrking med høyt innhold bildebehandling for å visualisere og kvantifisere multiple cellulære prosesser og strukturer i en high-throughput måte. Slike metoder kan hjelpe i den videre funksjonell annotering av genomer samt identifisere sykdom genet nettverk og potensielle narkotika mål.
Tags
High Content ScreeningNeurodegenerative DiseasesFunctional AnnotationGenomesMolecular NetworksDrug Target IdentificationGenetic Risk FactorsPathogenic MechanismsFunctional ValidationGenome Wide Association StudyParkinson’s DiseaseCausative VariantPathogenic MechanismAssociated RegionGenesPhenotypesTraditional Cell Biology TechniquesMolecular NetworksGenetic MutationsDisease PhenotypesNetwork ComplexityDrug Approval
