Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Morphometric Analyses of Retinal Sections
1Laboratory of Neurodegenerative Diseases, Department of Anatomy, LKS Faculty of Medicine,The University of Hong Kong, 2Research Centre of Heart, Brain, Hormone and Healthy Aging, LKS Faculty of Medicine,The University of Hong Kong, 3State Key Laboratory of Brain and Cognitive Sciences,The University of Hong Kong
Capítulos
- 00:01Título
- 00:20Introduction
- 01:01Measure the INL thickness
- 08:20Quantify the RGC number
- 08:34Differentiate live and dead cells
- 12:56Measure a cell’s size
- 14:10Conclusion
This video demonstrates three types of morphometric analyses of the retina, which include measuring the inner nuclear layer thickness, quantifying the number of retinal ganglion cells (RGCs) and measuring the sizes of RGCs. The technique can offer a simple but scientific platform for morphometric analyses.
Tags
Morphometric AnalysesRetinal SectionsRetinal DiseasesNeuronal CellsRetinal Ganglion Cell Layer (RGCL)N-methyl-D-aspartate (NMDA)-induced ExcitotoxicityRetinal Ischemia-reperfusion InjuryGlaucomaINL ThicknessInner Plexiform Layer (IPL) ThicknessCiticoline TreatmentKainic Acid-mediated Glutamate ExcitotoxicityRGC DensitySoma SizesElevated Intraocular PressureObjective MethodsEvaluating Retinal PathologiesTherapeutic StrategiesRetinal StructureMulti-layersDifferent Kinds Of NeuronsMorphometric ParametersCell NumberCell SizeThickness Of Different LayersCommercial Imaging SoftwarePrecise ValueAccurate CalculationChronic Glaucoma Model
