Journal
/
Biología
/
Single Cell transkriptionel Profilering af Voksen Mouse cardiomyocytter
JoVE Revista
Biología
This content is Free Access.
JoVE Revista Biología
Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes

Single Cell transkriptionel Profilering af Voksen Mouse cardiomyocytter

DOI:
10.3791/3302-v

•

08:23 min

•

December 28, 2011

•

, ,
1Buck Institute for Research on Aging, 2Department of Physiology & Biophysics,University of Washington

Capítulos

  • 00:05Título
  • 01:15Isolating Cardiomyocyte
  • 03:37Isolating Single Cells
  • 04:08Single Cell qPCR
  • 05:26Whole Transcriptome Amplification and Microarray of Single Cells
  • 07:00Quality Control Results
  • 07:56Conclusion

Summary

Traducción Automática

Traducción Automática

Enkelt celle udtryk profilering giver den detaljerede genekspression analyse af de enkelte celler. Vi beskriver metoder til isolering af cardiomyocytter, og forberede den resulterende lysates for enten hele transkriptom microarray eller qPCR af specifikke mål.

Tags

Single CellTranscriptional ProfilingAdult MouseCardiomyocytesGene ExpressionHomogenatesStochastic VariationPure Cardiomyocyte PopulationsCollagenase PerfusionMicroarraysWhole TranscriptomeQPCRNanofluidic ArraysGene Expression ProfilesEvaluation MetricsVariance Between CellsRobust AmplificationDouble Stranded CDNANimbleGen ArraysReverse Transcriptase – Specific Target Amplification (RT-STA)Nanofluidic PCRVariability Of Expression

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

check_url/es/3302?article_type=v

Generation of Single-Cell Suspensions from Mouse Neural Tissue
check_url/es/3302?article_type=v

Isolering og dyrkning af Neonatal Mouse cardiomyocytes
check_url/es/3302?article_type=v

Isolering og Fysiologisk Analyse af Mouse cardiomyocytter
check_url/es/3302?article_type=v

Isolering og dyrkning af Voksen Mouse kardiomyocytter for cellesignalering og<em> In vitro</em> Hjertehypertrofi
check_url/es/3302?article_type=v

Laser-assisteret mikrodissektion (LAM) som værktøj for transskriptionsprofilering af enkelte celle Typer
check_url/es/3302?article_type=v

Isolation, Kultur og transduktion af Voksen Mouse cardiomyocytter
check_url/es/3302?article_type=v

Trans-indre celle masse injektion af stamceller fra embryoner fører til højere kimærisme
check_url/es/3302?article_type=v

Droplet stregkodesystem-baserede enkelt celle Transcriptomics af voksne pattedyr væv
check_url/es/3302?article_type=v

Celle type-specifik genekspression profilering i muse leveren
check_url/es/3302?article_type=v

Isolation, transfektion og langsigtet kultur af voksne mus og rat kardiomyocytter

Read Article