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Hücre tipi siRNA teslimat için özel anti-HIV gp120 aptamers Geliştirme
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery
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Hücre tipi siRNA teslimat için özel anti-HIV gp120 aptamers Geliştirme

19,342 Views

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13:47 min

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June 23, 2011

DOI:

10.3791/2954-v

DOI:
10.3791/2954-v

•

13:47 min
June 23, 2011

•

5 Views
, , , ,
1Department of Molecular and Cellular Biology,Beckman Research Institute of City of Hope, 2Graduate School of Biological Sciences,Beckman Research Institute of City of Hope, 3Shared Resource-DNA/RNA Peptide,Beckman Research Institute of City of Hope

Summary

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Nanomole afinite ile HIV 1BA-L gp120 karşı çeşitli 2'-Fluoro RNA aptamers bir RNA kütüphanesi ile izole edilmiştir.<em> In vitro</em> SELEX prosedürü. Chimera yeni bir çift inhibitör fonksiyonu anti-gp120 aptamer-siRNA oluşturulur ve sistemik anti-HIV tedavisi için önemli bir söz gösterir.

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Zhou, J., Li, H., Zhang, J., Piotr, S., Rossi, J. Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery. J. Vis. Exp. (52), e2954, doi:10.3791/2954 (2011).

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