Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Single Drosophila Ommatidium Dissection and Imaging
13,867 Views
•
05:16 min
•
August 19, 2011
DOI:
Summary
Automatically generated
The limiting factor in the use of the adult Drosophila eye to study neurodegeneration and cell biology is the difficult imaging of intracellular processes. We describe the dissection of single ommatidia to generate a bona-fide primary neuronal cell culture, which can be subject to drug treatment and advanced imaging.
Explore More Videos
Drosophila MelanogasterFruit FlyNeuroscience ResearchNeurodegenerative DiseasesGenéticaFly EyeNeurodegeneration ResearchAutofluorescenceIntracellular EventsAutophagy DynamicsSingle Ommatidia DissectionDentatorubro-Pallidoluysian Atrophy (DRPLA)Technique DescriptionElectrophysiological StudiesFly Models For NeurodegenerationSubcellular Events ImagingDrug AdministrationPhotoreceptor CellsMosaic Techniques
Read Article
Cite this Article
Volpi, V., Mackay, D., Fanto, M. Single Drosophila Ommatidium Dissection and Imaging. J. Vis. Exp. (54), e2882, doi:10.3791/2882 (2011).
Copy