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Rapid Diagnosis of Avian Influenza Virus in Wild Birds: Use of a Portable rRT-PCR and Freeze-dried Reagents in the Field
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Rapid Diagnosis of Avian Influenza Virus in Wild Birds: Use of a Portable rRT-PCR and Freeze-dried Reagents in the Field
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August 02, 2011
DOI:
1USGS Western Ecological Research Center, 2Wildlife Health Center,University of California, Davis, 3Department of Population Health and Reproduction,University of California, Davis, 4Department of Veterinary and Biomedical Sciences,University of Minnesota , 5Science Applications International Corporation
Summary
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This study describes diagnosis of avian influenza in wild birds using a portable rRT-PCR system. The method takes advantage of freeze-dried reagents to screen wild birds in a non-laboratory setting, typical of an outbreak scenario. Use of molecular tools provides accurate and sensitive alternatives for rapid diagnosis.
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Takekawa, J. Y., Hill, N. J., Schultz, A. K., Iverson, S. A., Cardona, C. J., Boyce, W. M., Dudley, J. P. Rapid Diagnosis of Avian Influenza Virus in Wild Birds: Use of a Portable rRT-PCR and Freeze-dried Reagents in the Field. J. Vis. Exp. (54), e2829, doi:10.3791/2829 (2011).
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