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유전과 Epigenetic 분석을위한 파라핀 임베디드 재료에서 DNA 추출
JoVE Revista
Biología
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JoVE Revista Biología
DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses
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유전과 Epigenetic 분석을위한 파라핀 임베디드 재료에서 DNA 추출

55,371 Views

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13:32 min

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March 26, 2011

DOI:

10.3791/2763-v

DOI:
10.3791/2763-v

•

13:32 min
March 26, 2011

•

28 Views
, , ,
1Department of Integrative Oncology,BC Cancer Research Centre, 2Interdisciplinary Oncology Program,University of British Columbia – UBC, 3Photography/Video Production, Multi-Media Services,BC Cancer Agency, 4Department of Pathology and Laboratory Medicine,University of British Columbia – UBC

Summary

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이 동영상은 포르말린 고정 파라핀 - - 임베디드 물질로부터 DNA 추출을위한 프로토콜을 보여줍니다. 이것은 조직 섹션 크실렌과 deparaffinized하는 멀티 일 절차, 에탄올과 rehydrated 이후 유전자의 특정 또는 게놈 차원의 분석을 위해 DNA를 정화하고 분리 proteinase K로 처리됩니다.

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Pikor, L. A., Enfield, K. S. S., Cameron, H., Lam, W. L. DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses. J. Vis. Exp. (49), e2763, doi:10.3791/2763 (2011).

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