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V3的HIV - 1取向的基因型推理使用人口为基础的测序
JoVE Revista
Inmunología y contagio
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JoVE Revista Inmunología y contagio
Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3
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V3的HIV - 1取向的基因型推理使用人口为基础的测序

11,953 Views

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11:10 min

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December 27, 2010

DOI:

10.3791/2531-v

DOI:
10.3791/2531-v

•

11:10 min
December 27, 2010

•

3 Views
, ,
1Laboratory Program,BC Centre for Excellence in HIV/AIDS

Summary

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可以推断出艾滋病毒嗜性病毒包膜V3区。 V3是一式三份，采用巢式RT - PCR扩增，测序，PCR扩增，并解释使用生物信息学软件。被列为非R5病毒与G2P分数低1个或多个序列（S）的样品。

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Cite this Article

McGovern, R. A., Harrigan, P. R., Swenson, L. C. Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3. J. Vis. Exp. (46), e2531, doi:10.3791/2531 (2010).

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