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Arabidopsis thaliana Polar Glycerolipid Profiling by Thin Layer Chromatography (TLC) Coupled with Gas-Liquid Chromatography (GLC)
 

Arabidopsis thaliana Polar Glycerolipid Profiling by Thin Layer Chromatography (TLC) Coupled with Gas-Liquid Chromatography (GLC)

Article DOI: 10.3791/2518-v • 13:03 min • March 18th, 2011
March 18th, 2011 •
1, 1
1Department of Biochemistry and Molecular Biology, Michigan State University

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Composition of polar lipid extracts and the fatty acid composition of individual glycerolipids are determined in a simple and robust lipid profiling experiment. For this purpose, glycerolipids are isolated by thin layer chromatography and subjected to transmethylation of their acyl groups. Fatty acyl methylesters are quantified by gas-liquid chromatography.

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Arabidopsis Thaliana Polar Glycerolipid Profiling Thin Layer Chromatography (TLC) Gas-liquid Chromatography (GLC) Biological Membranes Glycerolipids Phosphatidylcholine Phosphatidylethanolamine Bilayer Membranes Chemical Exchange Chloroplast Glycolipids Monogalactosyldiacylglycerol (MGDG) Digalactosyldiacylglycerol (DGDG) Sulfoquinovosyldiacylglycerol (SQDG) Photosynthesis Crystal Structures Photosystem I And II Phosphate Starvation Extraplastidic Membranes Phospholipids Biosynthesis Genetic Studies Biochemical Studies
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