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March 11, 2011
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一个细胞类型特异性的蛋白定位和穿梭核质特性的灵活和有效的方法是描述。这异核体的方法是使用荧光标记的融合蛋白细胞融合后图像蛋白质本地化。该协议是经得起稳态本地化或活细胞成像的基础上更具活力的测定。
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Heterokaryon TechniqueCell Type-specific LocalizationSubcellular TraffickingNucleocytoplasmic ShuttlingProteinsCellular FunctionsNuclear Import/exportTranscriptional RegulationApoptosisCell DivisionDifferentiationTransformationProtein ActivitiesCellular OncogenesisNormal Primary CellsTransformed CellsAltered LocalizationPathwaysDrug TargetingProtein TraffickingShuttling ActivityMammalian CellsHeterokaryon FusionsFluorescence Microscopy
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Gammal, R., Baker, K., Heilman, D. Heterokaryon Technique for Analysis of Cell Type-specific Localization. J. Vis. Exp. (49), e2488, doi:10.3791/2488 (2011).
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