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Capítulos
- 00:00Título
- 00:21Subscription Link
- 02:52Introduction
- 03:31Preparing Nematoda Growth Media Plates for Paralysis Assay
- 05:34Preparation of Age-synchronous Worm Populations
- 08:04Transgene Induction and Paralysis Assay
- 11:48Representative Results
- 13:05Conclusion
The intensely studied nematode worm Caenorhabditis elegans can be transgenically engineered to express the human β-amyloid peptide (Aβ). Induced expression of Aβ in C. elegans muscle leads to a rapid, reproducible paralysis phenotype that can be used to monitor treatments that modulate Aβ toxicity.
Tags
Assayingβ-amyloid ToxicityTransgenic C. Elegans ModelAlzheimer’s DiseaseToxicity MechanismInclusion Body MyositisNematode WormCaenorhabditis ElegansTransgenic EngineeringAβ ExpressionMeasurable PhenotypesAssociative LearningParalysis PhenotypeTemperature-sensitive MutationMRNA Surveillance SystemTemperature-inducible Muscle ExpressionAβ TransgeneProtective TransgeneGinkgo Biloba Extracts
