Differentiating Human Embryonic Stem Cells into Neural Progenitors

Plate the cells on a basement membrane matrix coated 6-well plate at a density of 2 x 10⁵ cells per well in 2 milliliters of mTeSR1 with 10 micromolar ROCK inhibitor. After 24 hours, replace the culture medium with neural induction medium supplemented with 1 micromolar dorsomorphin and 5 micromolar SB431542. Change the medium every other day during the first four days of neural induction, then every day, until confluence is reached at day seven.