Journal
/
Neurociencias
/
Double Fluorescence in situ Hybridization in Fresh Brain Sections
JoVE Revista
Neurociencias
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Neurociencias
Double Fluorescence in situ Hybridization in Fresh Brain Sections

Double Fluorescence in situ Hybridization in Fresh Brain Sections

DOI:
10.3791/2102-v

•

12:15 min

•

August 14, 2010

•

, , ,
1Department of Brain and Cognitive Sciences,University of Rochester, 2Center for Visual Science,University of Rochester

Capítulos

  • 00:04Título
  • 00:07Introduction
  • 00:56Prep of Sephadex G-50 Columns
  • 02:54Labeling and Purification of Riboprobes
  • 04:25Post-fixation, Acetylation, and Hybridization
  • 07:22Detection and Visualization of Riboprobes
  • 10:35Representative Results
  • 11:35Conclusion

Summary

Traducción Automática

Traducción Automática

This protocol involves a non-radioactive in-situ hybridization procedure that enables the simultaneous identification of two transcript species, at a single cell resolution, in thin sections of the vertebrate brain.

Tags

Double Fluorescence In Situ HybridizationDFISHMRNAFresh Frozen Brain SectionsGene Co-regulationAnatomical OrganizationNeurochemical PropertiesSensory ExperienceCentral Sensory CircuitsSingle Cell ResolutionProtocolValidationMiceRatsSongbirdsVertebrate Species

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Combining Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology

Morphometric Analyses of Retinal Sections

Design and Assembly of an Ultra-light Motorized Microdrive for Chronic Neural Recordings in Small Animals

Complete Spinal Cord Injury and Brain Dissection Protocol for Subsequent Wholemount In Situ Hybridization in Larval Sea Lamprey

Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization

Rapid In Situ Hybridization using Oligonucleotide Probes on Paraformaldehyde-prefixed Brain of Rats with Serotonin Syndrome

Combining Double Fluorescence In Situ Hybridization with Immunolabelling for Detection of the Expression of Three Genes in Mouse Brain Sections

Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization

Isolation of Cerebral Capillaries from Fresh Human Brain Tissue

Double Direct Injection of Blood into the Cisterna Magna as a Model of Subarachnoid Hemorrhage

Read Article