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Electroporation Procedure for the Single-Copy Gene Expression in an Efflux-Deficient Bacterial Strain

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Inmunología y contagio

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JoVE Revista Inmunología y contagio

Electroporation Procedure for the Single-Copy Gene Expression in an Efflux-Deficient Bacterial Strain

Electroporation Procedure for the Single-Copy Gene Expression in an Efflux-Deficient Bacterial Strain

DOI:

10.3791/201442-v

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03:04 min

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January 05, 2024

•

Dawn White, Ayush Kumar

¹Department of Microbiology,University of Manitoba

Tags

Electroporation PTNS2 Helper Plasmid PUC18-Tmini-Tn7-Tlac Gentamicin AdeIJK Insertion Plasmid Electrocompetent Cells LB Broth LB Agar Gentamicin Colony PCR ABglmS2 Forward Tn7 Reverse A.baumannii ATCC 17978 AB 258

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